Anti-STEAP1 Antibody

Rabbit polyclonal antibody to STEAP1

Western blot analysis of STEAP1 expression in U87MG (A) whole cell lysates.
Immunohistochemical analysis of STEAP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9UHE8
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	39851 Da

Additional Information
Gene ID	26872
Other Names	PRSS24; STEAP; Metalloreductase STEAP1; Six-transmembrane epithelial antigen of prostate 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human STEAP1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	STEAP1
Synonyms	PRSS24, STEAP
Function	Does not function as a metalloreductase due to the absence of binding sites for the electron-donating substrate NADPH. Promotes Fe(3+) reduction when fused to the NADPH-binding domain of STEAP4.
Cellular Location	Endosome membrane {ECO:0000250\|UniProtKB:Q9CWR7}; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Tissue Location	Ubiquitously expressed. Highly expressed in prostate tumors.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.