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>   首页   >   产品   >   一抗   >   Druggable G protein-coupled receptors (GPCRs)   >   HTR1E Antibody (C-Term)   

HTR1E Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HTR1E Antibody (C-Term) AW5621
    All lanes : Anti-HTR1E Antibody (C-Term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: human brain lysate Lane 3: Jurkat whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 2 - HTR1E Antibody (C-Term) AW5621
    AW5619 staining HTR1E in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC, WB
Primary Accession P28566
Other Accession Q6VB83, Q9N2B6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 41682 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 3354
Antigen Region 223-258 aa
Other Names 5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E
Dilution IHC~~1:100~500
WB~~0.25
Target/Specificity This HTR1E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 223-258 amino acids from human HTR1E.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHTR1E Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HTR1E (HGNC:5291)
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:14744596, PubMed:1513320, PubMed:1608964, PubMed:1733778, PubMed:21422162, PubMed:33762731). Also functions as a receptor for various alkaloids and psychoactive substances (PubMed:14744596, PubMed:1513320, PubMed:1608964, PubMed:1733778, PubMed:21422162, PubMed:33762731). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:14744596, PubMed:1513320, PubMed:1608964, PubMed:1733778, PubMed:21422162, PubMed:33762731). HTR1E is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission by inhibiting adenylate cyclase activity (PubMed:33762731, PubMed:35610220).
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Detected in brain..
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.

REFERENCES

McAllister G.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:5517-5521(1992).
Levy F.O.,et al.FEBS Lett. 296:201-206(1992).
Zgombick J.M.,et al.Mol. Pharmacol. 42:180-185(1992).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).

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