Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) (Pre-Adsorbed) Secondary Antibody
Donkey Polyclonal, Alkaline Phosphatase (Calf Intestine)
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Description | Anti-CHICKEN IgG (H&L) (DONKEY) Antibody Alkaline Phosphatase Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) |
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Host | Donkey |
Conjugate | Alkaline Phosphatase (Calf Intestine) |
Target Species | Chicken |
Reactivity | Chicken |
Clonality | Polyclonal |
Application ![]()
| WB |
Physical State | Liquid (sterile filtered) |
Host Isotype | IgG |
Target Isotype | IgG (H&L) |
Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
Immunogen | Chicken IgG whole molecule |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.1% (w/v) Sodium Azide |
Shipping Condition | Wet Ice |
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Application Note | Anti-Chicken IgG (H&L) anitbody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-Chicken IgG (H&L) Antibody detects specifically Chicken IgG whole molecule. Anti-Chicken IgG (H&L) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Chicken IgG (H&L) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.

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