Anti-CLDN6 Reference Antibody (IMAB027)

Recombinant Antibody

Anti-CLDN6 Reference Antibody (IMAB027) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CLDN6 Reference Antibody (IMAB027)is more than 98.41% ,determined by SEC-HPLC.
Immobilized human CLDN6 VLP at 8 µg/mL can bind Anti-CLDN6 Reference Antibody (IMAB027)，EC50=0.001599 µg/mL
Human CLDN6 CHO cells were stained with Anti-CLDN6 Reference Antibody (IMAB027) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC199=4.342 nM
Anti-CLDN6 Reference Antibody (IMAB027)-induced ADCC activity was evaluated using Human CD16a (158V) (Luc) Jurkat Reporter Cell. The max induction fold was approximately 14.16
Anti-CLDN6 Reference Antibody (IMAB027) by huCLDN6-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 1 nM.
IMAB027 inhibited the tumor growth of OV90 on Balb/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 50.7% at 5 mpk at D34.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P56747
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	23292 Da

Additional Information
Target/Specificity	CLDN6
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CLDN6
Function	Plays a major role in tight junction-specific obliteration of the intercellular space.
Cellular Location	Cell junction, tight junction {ECO:0000250\|UniProtKB:Q9Z262}. Cell membrane; Multi-pass membrane protein
Tissue Location	Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.