Anti-CDCP1 / CD318 Reference Antibody (38 E11)

Recombinant Antibody

Anti-CDCP1 / CD318 Reference Antibody (38 E11) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CDCP1 / CD318 Reference Antibody (38 E11)is more than 95.48% ,determined by SEC-HPLC.
Immobilized human CDCP1 His at 2 µg/mL can bind Anti-CDCP1 / CD318 Reference Antibody (38 E11)，EC50=0.002496 µg/mL
Human CDCP1 HEK293 cells were stained with Anti-CDCP1 / CD318 Reference Antibody (38 E11) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC152=0.385 µg/mL

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q9H5V8
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	92932 Da

Additional Information
Target/Specificity	CDCP1 / CD318
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CDCP1
Synonyms	TRASK
Function	May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Cellular Location	[Isoform 1]: Cell membrane; Single- pass membrane protein. Note=Shedding may also lead to a soluble peptide
Tissue Location	Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.