Anti-TPBG Reference Antibody (PF-06263507)

Recombinant Antibody

Anti-TPBG Reference Antibody (PF-06263507) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-TPBG Reference Antibody (PF-06263507)is more than 99.25% ,determined by SEC-HPLC.
Immobilized human 5T4 His at 2 µg/mL can bind Anti-TPBG Reference Antibody (PF-06263507)，EC50=0.004604 µg/mL
Human 5T4 HEK293 cells were stained with Anti-TPBG Reference Antibody (PF-06263507) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC121=0.7286 ug/Ml

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q13641
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	46032 Da

Additional Information
Target/Specificity	TPBG
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	TPBG
Synonyms	5T4
Function	May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.