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Prostate Specific Antigen Mouse mAb

Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (AP94768) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Sample: Lane 1: Prostate Specific Antigen (PSA) Protein at 500ng Primary: Mouse Anti-PSA Monoclonal Antibody at 1/1000 dilution (Cat. AP94768) Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 34kD Observed band size: 32kD
Measured by its binding ability in a indirect ELISA. Immobilized Human PSA (Native) at 2 µg/mL (100 µL/well) can bind Anti-Human PSA monoclonal antibody, the minimum detection concentration is 0.1ng/mL
Sample: Lane 1: Human Prostate Specific Antigen protein Primary: Anti-Prostate Specific Antigen (AP94768) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kDa Observed band size: 35 kDa
Paraformaldehyde-fixed, paraffin embedded (Human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (AP94768) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Host	Rabbit
Clonality	Monoclonal
Calculated MW	23 KDa
Physical State	Liquid
Immunogen	Prostate Specific Antigen (PSA) Antigen, Native
Isotype	IgG1
Purity	affinity purified by Protein A
Buffer	0.01M PBS (pH7.4).
SUBCELLULAR LOCATION	Secreted.
SIMILARITY	Belongs to the peptidase S1 family. Kallikrein subfamily.Contains 1 peptidase S1 domain.
SUBUNIT	Forms heterodimer with SERPINA5.
Post-translational modifications	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Additional Information
Target/Specificity	Prostate.
Dilution	WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol