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C6ORF199 Rabbit pAb

C6ORF199 Rabbit pAb

     
  • 1 - C6ORF199 Rabbit pAb AP94755
    Sample:HepG2 (Human)CellLysate t 40 ug Primary: Anti-C6ORF199(AP94755)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 221kD Observed band size: 245kD
  • 14 - C6ORF199 Rabbit pAb AP94755
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C6ORF199) Polyclonal Antibody, Unconjugated (AP94755) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Primary Accession Q6ZNF1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 221 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human C6ORF199
Epitope Specificity 1601-1700/1911
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY Belongs to the adenylate kinase family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions AKD1 is a 1,911 amino acid coiled-coil protein belonging to the adenylate kinase family. AKD1 exists as six alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 6q21. Chromosome 6 makes up nearly 6% of the human genome and contains 170 million base pairs, which encode 1,200 genes. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. A bipolar disorder susceptibility locus is also linked to the q arm of chromosome 6. The PARK2 gene, which is associated with Parkinson's disease, and the genes encoding the major histocompatiblity complex proteins are located on chromosome 6. Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6.
Additional Information
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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