CD80 Rabbit pAb

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Raji (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Primary: Anti-CD80 (bs-1479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-65 kD Observed band size: 60 kD
Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Raji (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Primary: Anti-CD80 (AP94750) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-65 kD Observed band size: 60 kD

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	30 KDa
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from rat CD80
Epitope Specificity	251-321/321
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Membrane; Single-pass type I membrane protein.
SIMILARITY	Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

Additional Information
Target/Specificity	Expressed on activated B-cells, macrophages and dendritic cells.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol