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Regucalcin Rabbit pAb

Regucalcin Rabbit pAb

     
  • 14 - Regucalcin Rabbit pAb AP94749
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Regucalcin) Polyclonal Antibody, Unconjugated (AP94749) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 1 - Regucalcin Rabbit pAb AP94749
    Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Regucalcin (AP94749) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
  • 1 - Regucalcin Rabbit pAb AP94749
    Blank control(black line):HepG2. Primary Antibody (green line): Rabbit Anti-Regucalcin antibody (AP94749) Dilution:2ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • 14 - Regucalcin Rabbit pAb AP94749
    Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Regucalcin) Polyclonal Antibody, Unconjugated (AP94749) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - Regucalcin Rabbit pAb AP94749
    Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Regucalcin) Polyclonal Antibody, Unconjugated (AP94749) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - Regucalcin Rabbit pAb AP94749
    Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Regucalcin) Polyclonal Antibody, Unconjugated (AP94749) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - Regucalcin Rabbit pAb AP94749
    Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Regucalcin) Polyclonal Antibody, Unconjugated (AP94749) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Primary Accession Q5JRR5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 33 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human Regucalcin
Epitope Specificity 65-160/299
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm.
SIMILARITY Belongs to the SMP-30/CGR1 family.
SUBUNIT Monomer.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Regucalcin is a Ca2+ binding protein that is encoded by a highly conserved gene in vertebrate species. Regucalcin is greatly expressed in liver and kidney cortex, and the expression is mediated through a Ca2+ signaling mechanism with AP1 and NFI-A1 as transcriptional factors. It plays a multifunctional role as a regulatory protein in intracellular signaling processes in the cytoplasm and nucleus of cells including the maintenance of intracellular Ca2+ homeostasis, the regulation of various Ca2+ dependent protein kinases and tyrosine kinases, protein phosphatases, nitric oxide (NO) synthase, and the suppression of nuclear DNA and RNA syntheses. Regucalcin has suppressive effects on cell proliferation, cell death and apoptosis, and is suggested to play a physiologic role in the maintenance of cellular homeostasis.
Additional Information
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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