STOML2 Recombinant Mouse mAb

Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for AP94491
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: JK, 2: MCF-7, 3: Rat Brain, 4: N2a Protein loading quantity: 20 µg Exposure time: 3 s Predicted MW: 39 kDa Observed MW: 39 kDa

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, ICC
Host	Rabbit
Clonality	Recombinant
Physical State	Liquid
Isotype	IgG2b/Kappa
Purity	affinity purified by Protein G
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Membrane; peripheral membrane protein. Cytoplasm. Associated with the cytoskeleton.
SIMILARITY	Belongs to the band 7/mec-2 family.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	STOML2 is a 356 amino acid member of the mec-2 family of proteins. Expressed ubiquitously at low levels, STOML2 is highly expressed in heart, liver and pancreas. STOML2 is localized to the cytoplasm with some distribution on the membrane. STOML2 was first identified as an overexpressed protein in human endometrial adenocarcinoma. Changes in cell growth in samples with different levels of STOML2 indicate that STOML2 could play a role in endometrial tumorigenesis. STOML2 is also thought to play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.

Additional Information
Target/Specificity	Ubiquitously expressed at low levels.
Dilution	WB=1:1000-1:10000,ICC/IF=1:50
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.