LPCAT1 Recombinant Mouse mAb

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 4℃ overnight Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: Mouse spleen Protein loading quantity: 20 µg Exposure time: 30 s Predicted MW: 55 kDa Observed MW: 55 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: room temperature 2h Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: A431, A549 Protein loading quantity: 20 µg Exposure time: 10 s Predicted MW: 55 kDa Observed MW: 55 kDa

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Host	Rabbit
Clonality	Recombinant
Calculated MW	59 KDa
Physical State	Liquid
Isotype	IgG2b/Kappa
Purity	affinity purified by Protein G
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Endoplasmic reticulum membrane. Golgi apparatus membrane.
SIMILARITY	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Lysophosphatidylcholine (LPC) acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis (Nakanishi et al., 2006 [PubMed 16704971]).[supplied by OMIM, May 2008]

Additional Information
Dilution	WB=1:500-1:1000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.