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ASH1L Rabbit pAb

ASH1L Rabbit pAb

     
  • 14 - ASH1L Rabbit pAb AP94295
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT2H/ASH1L Polyclonal Antibody, Unconjugated(AP94295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF
Primary Accession Q9NR48
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 332790 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human ASH1L
Epitope Specificity 1985-2060/2969
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus. Cell junction, tight junction. Chromosome (Probable). Note=The relevance of tight junction localization is however unclear.
SIMILARITY Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 3 A.T hook DNA-binding domains.Contains 1 AWS domain. Contains 1 BAH domain. Contains 1 bromo domain. Contains 1 PHD-type zinc finger. Contains 1 post-SET domain. Contains 1 SET domain.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions ASH1L is a 2,969 amino acid protein encoded by the human gene ASH1L. ASH1L belongs to the histone-lysine methyltransferase family (SET2 subfamily) and contains three AT hook DNA-binding domains, one AWS domain, one BAH domain, one bromodomain, one PHD-type zinc finger, one post-SET domain and one SET domain. It is a widely expressed nuclear protein with highest expression found in brain, heart and kidney. ASH1L is a histone methyltransferase and is believed to methylate 'Lys-4' of Histone H3, which is a specific tag for epigenetic transcriptional activation.
Additional Information
Gene ID 55870
Other Names Histone-lysine N-methyltransferase ASH1L, 2.1.1.359, 2.1.1.367, ASH1-like protein, huASH1, Absent small and homeotic disks protein 1 homolog, Lysine N-methyltransferase 2H, ASH1L, KIAA1420, KMT2H
Target/Specificity Widely expressed, with highest level in brain, heart and kidney.
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name ASH1L
Synonyms KIAA1420, KMT2H
Function Histone methyltransferase specifically trimethylating 'Lys- 36' of histone H3 forming H3K36me3 (PubMed:21239497). Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). The physiological significance of the H3K9me1 activity is unclear (By similarity).
Cellular Location Nucleus. Cell junction, tight junction. Chromosome. Note=The relevance of tight junction localization is however unclear.
Tissue Location Widely expressed, with highest level in brain, heart and kidney.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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