SH2D7 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SH2D7) Polyclonal Antibody, Unconjugated (AP94226) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	A6NKC9
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	49807 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SH2D7
Epitope Specificity	181-280/451
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
SUBUNIT	Contains 1 SH2 domain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	SH2D7 (SH2 Domain Containing 7) is a Protein Coding gene. An important paralog of this gene is SH2D2A.

Additional Information
Gene ID	646892
Other Names	SH2 domain-containing protein 7, SH2D7
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.