mouse Ly-6G/Ly-6C Rat mAb

Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(AP94106) at 1:200 overnight at 4°C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(AP94106) at 1:200 overnight at 4°C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, FC
Primary Accession	P35461
Reactivity	Mouse
Host	Rabbit
Clonality	Monoclonal
Calculated MW	14324 Da
Physical State	Liquid
Immunogen	Recombinant Mouse Ly-6G/Gr-1
Isotype	Rat IgG2b, κ
Purity	affinity purified by Protein G
Buffer	0.01M PBS.
SUBCELLULAR LOCATION	Cell membrane; Lipid-anchor, GPI-anchor (By similarity).
SIMILARITY	Contains 1 UPAR/Ly6 domain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

Additional Information
Gene ID	546644
Other Names	Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g
Target/Specificity	Expressed in bone marrow.
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.