AFP(A2) Mouse mAb
AFP(A2) Mouse mAb
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- 实验流程
- 背景知识
Application ![]()
| WB, IHC-P, IHC-F, IF, E |
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Host | Rabbit |
Clonality | Monoclonal |
Calculated MW | 65 KDa |
Physical State | Liquid |
Immunogen | AFP protein |
Epitope Specificity | full length |
Isotype | IgG |
Purity | affinity purified by Protein G |
Buffer | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
SUBCELLULAR LOCATION | Secreted. |
SIMILARITY | Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains. |
SUBUNIT | Dimeric and trimeric forms have been found in addition to the monomeric form. |
Post-translational modifications | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.Sulfated. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
Background Descriptions | Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. |
Target/Specificity | Plasma. Synthesized by the fetal liver and yolk sac. |
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Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
Format | 0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce |
Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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