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AFP(A2) Mouse mAb

AFP(A2) Mouse mAb

     
  • 10 - AFP(A2) Mouse mAb AP94036
    Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. bs-43140P) at 2 µg/mL (100 µL/well) can bind   Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/mL.
  • 1 - AFP(A2) Mouse mAb AP94036
    25 ug total protein per lane of various lysates (see on figure) probed with AFP(A2) monoclonal antibody, unconjugated (AP94036) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
  • 14 - AFP(A2) Mouse mAb AP94036
    (Negative control)Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFP(A2) Monoclonal Antibody, Unconjugated(AP94036) at 1:500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
  • 14 - AFP(A2) Mouse mAb AP94036
    (Negative control)Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFP(A2) Monoclonal Antibody, Unconjugated(AP94036) at 1:500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
  • 14 - AFP(A2) Mouse mAb AP94036
    Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFP(A2) Monoclonal Antibody, Unconjugated(AP94036) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF, E
Host Rabbit
Clonality Monoclonal
Calculated MW 65 KDa
Physical State Liquid
Immunogen AFP protein
Epitope Specificity full length
Isotype IgG
Purity affinity purified by Protein G
Buffer 0.01M PBS (pH7.4) with 0.02% Proclin300.
SUBCELLULAR LOCATION Secreted.
SIMILARITY Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.
SUBUNIT Dimeric and trimeric forms have been found in addition to the monomeric form.
Post-translational modifications Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.Sulfated.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.
Additional Information
Target/Specificity Plasma. Synthesized by the fetal liver and yolk sac.
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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