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C14orf80 (12B11) Mouse Monoclonal antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93910) (1:250)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93910) (1:250)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93910) (1:250)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93910) (1:250)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93910) (1:250)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C14orf80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C14orf80 (Cat# AP93910)(1:2000).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q86SX3
Reactivity	Human
Clonality	Monoclonal
Calculated MW	54231 Da

Additional Information
Gene ID	283643
Other Names	Tubulin epsilon and delta complex protein 1, TEDC1 (HGNC:20127)
Dilution	WB~~1:1000 IHC~~1:100~500
Storage Conditions	-20℃

Protein Information
Name	TEDC1 (HGNC:20127)
Function	Acts as a positive regulator of ciliary hedgehog signaling. Required for centriole stability (By similarity). May play a role in counteracting perturbation of actin filaments, such as after treatment with the actin depolymerizing microbial metabolite Chivosazole F (PubMed:28796488).
Cellular Location	Cell projection, cilium {ECO:0000250\|UniProtKB:Q3UK37}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250\|UniProtKB:Q3UK37}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol