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HSCB (11S6) Mouse Monoclonal antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, AP93906)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, AP93906)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSCB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, AP93906)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, AP93906)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, AP93906)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, AP93906)

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q8IWL3
Reactivity	Rat, Human, Mouse, Monkey, Dog
Clonality	Monoclonal
Calculated MW	27422 Da

Additional Information
Gene ID	150274
Other Names	Iron-sulfur cluster co-chaperone protein HscB, DnaJ homolog subfamily C member 20, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic, C-HSC20, Iron-sulfur cluster co-chaperone protein HscB, mitochondrial, HSCB (HGNC:28913), DNAJC20, HSC20
Dilution	WB~~1:1000 IHC~~1:100~500
Storage Conditions	-20℃

Protein Information
Name	HSCB (HGNC:28913)
Synonyms	DNAJC20, HSC20
Function	[Iron-sulfur cluster co-chaperone protein HscB, mitochondrial]: Acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria (PubMed:20668094). Required for incorporation of iron- sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain (PubMed:26749241). Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20 (PubMed:26749241). Plays an essential role in hematopoiesis (By similarity).
Cellular Location	[Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic]: Cytoplasm
Tissue Location	Expressed in lung, brain, stomach, spleen, ovary, testis, liver, muscle and heart.