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DUPD1 (DUSP27) (18A10) Mouse Monoclonal antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, AP93840)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, AP93840)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, AP93840)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, AP93840)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DUPD1 antibody (AP93840), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, AP93840)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, AP93840)

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q68J44
Reactivity	Human, Mouse
Clonality	Monoclonal
Calculated MW	25336 Da

Additional Information
Gene ID	338599
Other Names	Dual specificity phosphatase 29 {ECO:0000312\|HGNC:HGNC:23481}, Dual specificity phosphatase 27, Dual specificity phosphatase DUPD1, 3.1.3.16, 3.1.3.48, DUSP29 (HGNC:23481)
Dilution	WB~~1:1000 IHC~~1:100~500
Storage Conditions	-20℃

Protein Information
Name	DUSP29 (HGNC:23481)
Function	Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues within the same substrate, with a preference for phosphotyrosine as a substrate (PubMed:17498703). Involved in the modulation of intracellular signaling cascades. In skeletal muscle regulates systemic glucose homeostasis by activating, AMPK, an energy sensor protein kinase (By similarity). Affects MAP kinase signaling though modulation of the MAPK1/2 cascade in skeletal muscle promoting muscle cell differentiation, development and atrophy (By similarity).
Cellular Location	Cytoplasm. Nucleus {ECO:0000250\|UniProtKB:Q8BK84}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol