CK010 Rabbit Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC, IF
Primary Accession	P61165
Reactivity	Human, Mouse
Host	Polyclonal, Rabbit,IgG
Clonality	Polyclonal
Calculated MW	9079 Da

Additional Information
Gene ID	746
Other Names	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258, Oligosaccharyl transferase subunit TMEM258, Transmembrane protein 258, TMEM258 (HGNC:1164), C11orf10
Dilution	IHC~~1:100~500 IF~~1:50~200
Storage Conditions	-20℃

Protein Information
Name	TMEM258 (HGNC:1164)
Synonyms	C11orf10
Function	Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (PubMed:26472760, PubMed:27974209). Involved in ER homeostasis in the colonic epithelium (By similarity).
Cellular Location	Membrane; Multi- pass membrane protein. Endoplasmic reticulum. Cytoplasm
Tissue Location	Ubiquitously expressed.