C21orf29 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-C21orf29 Antibody (Center) at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with C21orf29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
C21orf29 Antibody (Center) (Cat. #AP9275c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB, E
Primary Accession	Q8WU66
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	74924 Da
Antigen Region	293-320 aa

Additional Information
Gene ID	54084
Other Names	Thrombospondin-type laminin G domain and EAR repeat-containing protein, TSP-EAR, TSPEAR, C21orf29
Target/Specificity	This C21orf29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-320 amino acids from the Central region of human C21orf29.
Dilution	IHC-P~~1:100~500 FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	C21orf29 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TSPEAR
Synonyms	C21orf29
Function	Plays a critical role in tooth and hair follicle morphogenesis through regulation of the Notch signaling pathway (PubMed:27736875). May play a role in development or function of the auditory system (PubMed:22678063).
Cellular Location	Secreted {ECO:0000250\|UniProtKB:J3S6Y1}. Cell surface {ECO:0000250\|UniProtKB:J3S6Y1}. Cell projection, stereocilium {ECO:0000250\|UniProtKB:J3S6Y1}. Note=Secreted protein which may bind to the cell surface via a membrane receptor {ECO:0000250\|UniProtKB:J3S6Y1}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.