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FCGR1B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-FCGR1B Antibody (C-term) at 1:500 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of AP9197b on paraffin-embedded human tonsil tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
FCGR1B Antibody (C-term) (Cat. #AP9197b) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P-Leica, E
Primary Accession	Q92637
Other Accession	A6NKC4, P12314
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	32232 Da
Antigen Region	248-276 aa

Additional Information
Other Names	High affinity immunoglobulin gamma Fc receptor IB, IgG Fc receptor IB, Fc-gamma RIB, FcRIB, hFcgammaRIB, FCGR1B, IGFRB
Target/Specificity	This FCGR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the C-terminal region of human FCGR1B.
Dilution	WB~~1:500 FC~~1:10~50 IHC-P-Leica~~1:100 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	FCGR1B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	FCGR1BP (HGNC:3614)
Synonyms	FCGR1B, IGFRB
Function	May bind to the Fc region of immunoglobulins gamma with a low affinity compared to FCGR1A. May function in the humoral immune response.
Cellular Location	Cell membrane; Single-pass type I membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

FCGR1B may bind to the Fc region of immunoglobulins gamma with a low affinity compared to FCGR1A. This protein may function in the humoral immune response.

REFERENCES

Thomas,G., et.al., Nat. Genet. 41 (5), 579-584 (2009)
Kuwano,Y., et.al., Arch. Dermatol. Res. 298 (10), 493-498 (2007)

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¥ 1,250.00

Cat# AP9197B

Size:

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100 µl

200 µl

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Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

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FCGR1B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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