Phospho-Cdk1/2 (T14) Antibody

Rabbit mAb

Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea.
Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP
Primary Accession	P06493/P24941
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	CDC28; CDC2A; CDK1; Cell division control protein 2 homolog; Cyclin-dependent kinase 1; MPF; kinase Cdc2; p34 protein kinase;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	34 KDa

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human Phospho-Cdk1/2 (T14)
Description	Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.