Cytochrome P450 2E1 Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P05181
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Immunogen	A synthesized peptide derived from human CYP2E1
Purification	Affinity Chromatography
Calculated MW	56849 Da

Additional Information
Gene ID	1571
Other Names	CYP2E1
Dilution	WB~~1/500-1/1000
Format	Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Protein Information
Name	CYP2E1 {ECO:0000303\|PubMed:10553002, ECO:0000312\|HGNC:HGNC:2631}
Function	A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10553002, PubMed:18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Microsome membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Mitochondrion inner membrane {ECO:0000250\|UniProtKB:P05182}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P05182}. Note=Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein {ECO:0000250\|UniProtKB:P05182}