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Anti-Vasopressin V2 Receptor Antibody

Rabbit polyclonal antibody to Vasopressin V2 Receptor

Western blot analysis of Vasopressin V2 Receptor expression in DLD (A), mouse muscle (B), rat kidney (C) whole cell lysates.
Immunofluorescent analysis of Vasopressin V2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P30518
Other Accession	O88721
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	40279 Da

Additional Information
Gene ID	554
Other Names	ADHR; DIR; DIR3; V2R; Vasopressin V2 receptor; V2R; AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor
Target/Specificity	Recognizes endogenous levels of Vasopressin V2 Receptor protein.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	AVPR2
Synonyms	ADHR, DIR, DIR3, V2R
Function	Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Kidney.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Vasopressin V2 Receptor. The exact sequence is proprietary.

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¥ 1,500.00

Cat# AP60541

Size:

50ul

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

Quantity:

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Availability: 3-5 days

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Anti-Vasopressin V2 Receptor Antibody

Rabbit polyclonal antibody to Vasopressin V2 Receptor

BACKGROUND

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