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AVPR2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

All lanes: Anti-AVPR2 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 45 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Jurkat cells stained with AP21193b (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P30518
Reactivity	Human
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	40279 Da

Additional Information
Gene ID	554
Other Names	Vasopressin V2 receptor, V2R, AVPR V2, Antidiuretic hormone receptor, Renal-type arginine vasopressin receptor, AVPR2, ADHR, DIR, DIR3, V2R
Target/Specificity	This AVPR2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 343-377 amino acids from the C-terminal region of human AVPR2.
Dilution	WB~~1:1000 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	AVPR2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	AVPR2
Synonyms	ADHR, DIR, DIR3, V2R
Function	Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Kidney.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.

REFERENCES

Seibold A.,et al.Am. J. Hum. Genet. 51:1078-1083(1992).
Birnbaumer M.,et al.Nature 357:333-335(1992).
Wildin R.S.,et al.Am. J. Hum. Genet. 55:266-277(1994).
Fay M.J.,et al.Peptides 17:477-481(1996).
North W.G.,et al.Cancer Res. 58:1866-1871(1998).

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¥ 1,250.00

Cat# AP21193b

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

询价

AVPR2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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