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Anti-ATP5H Antibody

Rabbit polyclonal antibody to ATP5H

     
  • 1 - Anti-ATP5H Antibody AP60540
    Western blot analysis of ATP5H expression in Hela (A), HGC27 (B) whole cell lysates.
  • 2 - Anti-ATP5H Antibody AP60540
    Immunohistochemical analysis of ATP5H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession O75947
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 18491 Da
Additional Information
Gene ID 10476
Other Names ATP synthase subunit d mitochondrial; ATPase subunit d
Target/Specificity Recognizes endogenous levels of ATP5H protein.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ATP5PD (HGNC:845)
Synonyms ATP5H
Function Subunit d, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed:37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed:37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed:37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed:37244256). Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements (By similarity).
Cellular Location Mitochondrion. Mitochondrion inner membrane.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATP5H. The exact sequence is proprietary.

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