Anti-CYB5R3 Antibody

Rabbit polyclonal antibody to CYB5R3

Western blot analysis of CYB5R3 expression in MCF7 (A), A549 (B), mouse testis (C), rat testis (D) whole cell lysates.
Immunohistochemical analysis of CYB5R3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IHC
Primary Accession	P00387
Reactivity	Human, Mouse, Rat, Drosophila
Host	Rabbit
Clonality	Polyclonal
Calculated MW	34235 Da

Additional Information
Gene ID	1727
Other Names	DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CYB5R3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100) IP~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	CYB5R3 (HGNC:2873)
Synonyms	DIA1
Function	Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Cellular Location	[Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250\|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250\|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250\|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250\|UniProtKB:P20070}
Tissue Location	[Isoform 2]: Expressed at late stages of erythroid maturation.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.