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Copine-6 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Copine-6 Polyclonal Antibody, Unconjugated(AP59296) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, ICC, E
Primary Accession	O95741
Reactivity	Rat, Pig
Host	Rabbit
Clonality	Polyclonal
Calculated MW	61991 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Copine-6
Epitope Specificity	221-320/557
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the copine family. Contains 2 C2 domains. Contains 1 VWFA domain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Copine 6 is a 557 amino acid member of the copine family of evolutionarily conserved, soluble, calcium-dependent, membrane-binding proteins. Members of the copine family are involved in signal transduction and membrane trafficking. Arabidopsis thaliana mutants lacking copine proteins exhibit reduced cell number and smaller cell size, effects which may be due to a defect in vesicle fusion or transport. Copine 6 contains two N-terminal C2 domains and one C-terminal VWFA (von Willebrand factor A) domain, which is also referred to as the A domain or the core domain. As is characteristic of the copine family, copine 6 functions in membrane trafficking and is capable of binding phospholipids in a calcium-dependent manner. Copine 6 may also play a role in synaptic plasticity.

Additional Information
Gene ID	9362
Other Names	Copine-6, Copine VI, Neuronal-copine, N-copine, CPNE6 (HGNC:2319)
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	CPNE6 (HGNC:2319)
Function	Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Binds phospholipid membranes in a calcium-dependent manner (By similarity). Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:Q9Z140}. Cell membrane {ECO:0000250\|UniProtKB:Q9Z140}. Endosome {ECO:0000250\|UniProtKB:Q9Z140}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000250\|UniProtKB:Q9Z140}. Perikaryon {ECO:0000250\|UniProtKB:Q9Z140}. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q9Z140}. Note=Mainly cytoplasmic in absence of calcium. Associated predominantly with membranes in presence of calcium. Translocates to the cell membrane in a calcium-dependent manner. Colocalized with transferrin in intracellular clathrin-coated membrane vesicles in a calcium-dependent manner {ECO:0000250\|UniProtKB:Q9Z140}
Tissue Location	Widely expressed in the brain (PubMed:10403379, PubMed:12949241, PubMed:9645480). Expressed weakly in the kidney, liver and fetal heart (PubMed:12949241). Expressed in melanocytes (PubMed:23999003).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol