ATP2B3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling ATP2B3 with AP21234b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on Hela cell line.The nuclear counter stain is DAPI (blue).
Anti-ATP2B3 Antibody (C-term)at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, E
Primary Accession	Q16720
Reactivity	Human, Rat
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	134197 Da

Additional Information
Gene ID	492
Target/Specificity	This ATP2B3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1161-1194 amino acids from the C-terminal region of human ATP2B3.
Dilution	WB~~1:2000 IF~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ATP2B3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ATP2B3 {ECO:0000303\|PubMed:8187550, ECO:0000312\|HGNC:HGNC:816}
Function	ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels at the presynaptic terminals (PubMed:18029012, PubMed:22912398, PubMed:25953895, PubMed:27035656). Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment (PubMed:25953895, PubMed:27035656). May counter-transport protons, but the mechanism and the stoichiometry of this Ca(2+)/H(+) exchange remains to be established (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Presynaptic cell membrane {ECO:0000250\|UniProtKB:Q64568}; Multi-pass membrane protein. Note=Localized at parallel fiber terminals {ECO:0000250\|UniProtKB:Q64568}
Tissue Location	Highly expressed in the cerebellum (PubMed:8187550). Expressed in adrenal glands (PubMed:27035656)