CO027 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

CO027 Antibody (C-term) (Cat. #AP10811b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CO027 antibody detected the CO027 protein (arrow).
CO027 Antibody (C-term) (Cat. #AP10811b) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the CO027 antibody detected the CO027 protein (arrow).
CO027 Antibody (C-term) (Cat. #AP10811b) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q2M3C6
Other Accession	Q9N0B5, NP_689548.2
Reactivity	Human, Mouse
Predicted	Monkey
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	57626 Da
Antigen Region	503-531 aa

Additional Information
Gene ID	123591
Other Names	Transmembrane protein C15orf27, C15orf27
Target/Specificity	This CO027 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-531 amino acids from the C-terminal region of human CO027.
Dilution	WB~~1:1000 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CO027 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TMEM266 (HGNC:26763)
Function	Voltage-sensor protein present on the post-synaptic side of glutamatergic mossy fibers and granule cells in the cerebellum (PubMed:25165868, PubMed:30810529). Despite the presence of a voltage- sensor segment, does not form a functional ion channel and its precise role remains unclear (PubMed:25165868, PubMed:30810529). Undergoes both rapid and slow structural rearrangements in response to changes in voltage (PubMed:30810529). Contains a zinc-binding site that can regulate the slow conformational transition (PubMed:30810529).
Cellular Location	Cell membrane; Multi-pass membrane protein. Cell projection, dendrite Perikaryon. Note=Present in the dendrites and soma of cerebellar granule neurons, but not in their axon
Tissue Location	Mainly expressed in the cerebellum (PubMed:25165868). Also expressed in cerebral cortex, skeletal muscle and thyroid, but at much lower levels (PubMed:25165868)