Mouse Monoclonal Antibody to CDCP1

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using CDCP1 mAb against human CDCP1 (AA: 692-836) recombinant protein. (Expected MW is 42 kDa)
Western blot analysis using CDCP1 mAb against HEK293 (1) and CDCP1 (AA: 692-836)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis of paraffin-embedded Hela tissues using CDCP1 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	Q9H5V8
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5G3H11
Isotype	Mouse IgG1
Calculated MW	92932 Da
Description	This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. ;
Immunogen	Purified recombinant fragment of human CDCP1 (AA: 692-836) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide
Application Note	ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000;

Additional Information
Gene ID	64866
Other Names	CD318; TRASK; SIMA135
Dilution	WB~~1:1000 IHC~~1:100~500 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Mouse Monoclonal Antibody to CDCP1 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CDCP1
Synonyms	TRASK
Function	May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Cellular Location	[Isoform 1]: Cell membrane; Single- pass membrane protein. Note=Shedding may also lead to a soluble peptide
Tissue Location	Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.