Phospho-Cdc25B (Ser323) Antibody

WB (WB) analysis using Phospho.Cdc25B (S323) Polyclonal Antibody in VEC, 293 and A549 cell lysates

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	P30305
Reactivity	Human, Mouse, Rat
Host	Rabbit
Isotype	Rabbit IgG
Calculated MW	64987 Da

Additional Information
Gene ID	994
Positive Control	WB: VEC, 293 and A549 cell lysates
Application & Usage	WB 1:500-1:2000; E 1:20000
Other Names	M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2
Target/Specificity	CDC25B
Antibody Form	Liquid
Appearance	Colorless liquid
Handling	The antibody solution should be gently mixed before use
Reconstitution & Storage	-20°C
Background Descriptions
Precautions	Phospho-Cdc25B (Ser323) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CDC25B
Synonyms	CDC25HU2
Function	Tyrosine protein phosphatase which functions as a dosage- dependent inducer of mitotic progression (PubMed:1836978, PubMed:20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed:20360007). Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner (PubMed:17332740). The three isoforms seem to have a different level of activity (PubMed:1836978).
Cellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole