Wnt-3a
- 产品详情
- 实验流程
Species | Human |
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Purity | > 95% as determined by Bis-Tris PAGE |
Endotoxin Level | Less than 1 EU per µg by the LAL method. |
Biological Activity | Wnt-3a hFc Chimera, Human (Cat.No.: Z03815) can induce Topflash reporter activity in HEK293T human embryonic kidney cells. |
Expression System | HEK293 |
Theoretical Molecular Weight | 58.50 kDa. |
Formulation | Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 µg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability | Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Target Background | Wnt-3a activates the canonical Wnt signaling pathway and is expressed in the dorsal midline region, playing a crucial role in spinal cord development. Additionally, it regulates autophagy, apoptosis, neuron regeneration, neurogenic inflammation, and axon regeneration. Wnt-3a promotes the beta-catenin/Tcf pathway, which can induce tumors and lead to cancer in specific cell populations. Moreover, it is widely used in cytokine for organoid construction. |
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Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.

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