癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PEF1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB |
|---|---|
| Primary Accession | Q9UBV8 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 30381 Da |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 553115 |
|---|---|
| Antigen Region | 95-121 aa |
| Other Names | Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32 |
| Dilution | WB~~1:1000 |
| Target/Specificity | This PEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the Central region of human PEF1. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PEF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PEF1 (HGNC:30009) |
|---|---|
| Synonyms | ABP32 |
| Function | Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity). |
| Cellular Location | Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q641Z8}. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+) (PubMed:11278427). Localizes to endoplasmic reticulum exit site (ERES) (By similarity). {ECO:0000250|UniProtKB:Q641Z8, ECO:0000269|PubMed:11278427} |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.
REFERENCES
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hansen, C., et al. FEBS Lett. 545 (2-3), 151-154 (2003) :
Satoh, H., et al. Biochim. Biophys. Acta 1600 (1-2), 61-67 (2002) :
Kitaura, Y., et al. Arch. Biochem. Biophys. 399(1):12-18(2002)
Kitaura, Y., et al. J. Biol. Chem. 276(17):14053-14058(2001)
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