CAPN2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

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Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody(cat. #AW5279). AW5279 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, Cat#AW5279) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody(Cat#AW5279). AW5279 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB
Primary Accession	P17655
Reactivity	Human, Rat
Predicted	Mouse
Host	Mouse
Clonality	monoclonal
Calculated MW	80 KDa
Isotype	IgG2b
Antigen Source	HUMAN

Additional Information
Other Names	Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
Dilution	IHC-P~~1:100~500 FC~~1:100 WB~~1:1000
Target/Specificity	This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CAPN2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

REFERENCES

Imajoh S.,et al.Biochemistry 27:8122-8128(1988).
Ye Z.,et al.Biochem. Biophys. Res. Commun. 275:223-227(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Hata A.,et al.J. Biol. Chem. 264:6404-6411(1989).

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¥ 2,600.00

Cat# AW5279

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: In Stock

询价

CAPN2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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