CAPN1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

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Western blot analysis of lysates from MCF-7,A549,A431 cell line (from left to right), using CAPN1 Antibody(Cat. #AW5228). AW5228 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Flow cytometric analysis of Hela cells using CAPN1 Antibody(green, Cat#AW5228) compared to an isotype control of mouse IgG1(blue). AW5228 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. kidney section using CAPN1 Antibody(Cat#AW5228). AW5228 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB
Primary Accession	P07384
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	82 KDa
Isotype	IgG1,k
Antigen Source	HUMAN

Additional Information
Other Names	Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, muCANP, CAPN1, CANPL1
Dilution	IHC-P~~1:100~500 FC~~1:25 WB~~1:1000
Target/Specificity	This CAPN1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN1.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CAPN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

REFERENCES

Aoki K.,et al.FEBS Lett. 205:313-317(1986).
Sorimachi H.,et al.Biol. Chem. Hoppe-Seyler 371:171-176(1990).
Kim J.W.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
Melloni E.,et al.Biochem. Biophys. Res. Commun. 229:193-197(1996).
Michetti M.,et al.FEBS Lett. 392:11-15(1996).

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¥ 2,600.00

Cat# AW5228

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

reduce add

Availability: In Stock

询价

CAPN1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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