> 首页 > 产品 > 一抗 > 神经科学 > SFN Antibody (C-term)

SFN Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

标注"皇冠"的产品，是满足高质量标准的产品。
了解更多...

Western blot analysis of lysates from A431,Hela cell line (from left to right), using SFN Antibody (C-term)(Cat. #AW5163). AW5163 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
SFN antibody (C-term) (Cat. #AW5163) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of SFN Antibody (C-term)(Cat. #AW5163) with A549 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
SFN Antibody (C-term) (Cat. #AW5163) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, IF, IHC-P, WB
Primary Accession	P31947
Other Accession	NP_006133.1
Reactivity	Mouse, Human
Predicted	Mouse, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	27774 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	2810
Antigen Region	222-248 aa
Other Names	SFN; HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin
Dilution	FC~~1:10~50 IF~~1:10~50 IHC-P~~1:100~500 WB~~1:1000
Target/Specificity	This SFN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-248 amino acids from the C-terminal region of human SFN.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SFN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SFN
Synonyms	HME1 {ECO:0000303\|PubMed:1390337}
Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127).
Cellular Location	Cytoplasm. Nucleus {ECO:0000250\|UniProtKB:O70456} Secreted. Note=May be secreted by a non- classical secretory pathway.
Tissue Location	Present mainly in tissues enriched in stratified squamous keratinizing epithelium.