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>   首页   >   产品   >   一抗   >   癌症   >   BCL10 Antibody   

BCL10 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - BCL10 Antibody AW5069
    Western blot analysis of lysates from Daudi,Raji cell line (from left to right), using BCL10 Antibody(Cat. #AW5069). AW5069 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 4 - BCL10 Antibody AW5069
    Flow cytometric analysis of Hela cells using BCL10 Antibody(green, Cat#AW5069) compared to an isotype control of mouse IgG1(blue). AW5069 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - BCL10 Antibody AW5069
    Immunohistochemical analysis of paraffin-embedded H. kidney section using BCL10 Antibody(Cat#AW5069). AW5069 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - BCL10 Antibody AW5069
    Immunohistochemical analysis of paraffin-embedded M. kidney section using BCL10 Antibody(Cat#AW5069). AW5069 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession O95999
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 26252 Da
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 8915
Antigen Region 1-143 aa
Other Names B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10, Bcl-10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, hCLAP, CED-3/ICH-1 prodomain homologous E10-like regulator, CIPER, Cellular homolog of vCARMEN, cCARMEN, Cellular-E10, c-E10, Mammalian CARD-containing adapter mol
Dilution IHC-P~~1:100~500
FC~~1:25
WB~~1:1000
Target/Specificity This BCL10 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-143 amino acids from the human region of human BCL10.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCL10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL10 {ECO:0000303|PubMed:9989495, ECO:0000312|HGNC:HGNC:989}
Function Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation (PubMed:10187770, PubMed:10364242, PubMed:10400625, PubMed:24074955, PubMed:25365219). Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:24074955). Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex (PubMed:24074955). This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:18287044, PubMed:24074955, PubMed:27777308). Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity (PubMed:26488816). Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR) (PubMed:18264101, PubMed:18287044, PubMed:24074955, PubMed:27777308). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK (PubMed:10187815).
Cellular Location Cytoplasm, perinuclear region. Membrane raft. Note=Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
Tissue Location Ubiquitous..
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.

REFERENCES

Willis T.G.,et al.Cell 96:35-45(1999).
Koseki T.,et al.J. Biol. Chem. 274:9955-9961(1999).
Thome M.,et al.J. Biol. Chem. 274:9962-9968(1999).
Yan M.,et al.J. Biol. Chem. 274:10287-10292(1999).
Srinivasula S.M.,et al.J. Biol. Chem. 274:17946-17954(1999).

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