癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
RNF168 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RNF168.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | Q8IYW5 |
| Other Accession | NM_152617 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG2b Kappa |
| Clone Names | 30 |
| Calculated MW | 65020 Da |
| Gene ID | 165918 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RNF168, hRNF168, 632-, RING finger protein 168, RNF168 |
| Target/Specificity | RNF168 (NP_689830, 462 a.a. ~ 571 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RNF168 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).
REFERENCES
53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair. Noon AT, et al. Nat Cell Biol, 2010 Feb. PMID 20081839.The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination. Ramachandran S, et al. Proc Natl Acad Sci U S A, 2010 Jan 12. PMID 20080757.A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses. Lilley CE, et al. EMBO J, 2010 Mar 3. PMID 20075863.RNF168, a new RING finger, MIU-containing protein that modifies chromatin by ubiquitination of histones H2A and H2AX. Pinato S, et al. BMC Mol Biol, 2009 Jun 5. PMID 19500350.RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Doil C, et al. Cell, 2009 Feb 6. PMID 19203579.
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