癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
RNF111 Antibody (monoclonal) (M05)
Mouse monoclonal antibody raised against a partial recombinant RNF111.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q6ZNA4 |
| Other Accession | NM_017610 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 1C4 |
| Calculated MW | 108862 Da |
| Gene ID | 54778 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase Arkadia, 632-, RING finger protein 111, RNF111 |
| Target/Specificity | RNF111 (NP_060080, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RNF111 Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein encoded by this gene contains a RING finger domain, a motif known to be involved in protein-protein and protein-DNA interactions. The mouse counterpart of this gene (Rnf111/arkadia) has been shown to genetically interact with the transforming growth factor (TGF) beta-like factor Nodal, and act as a modulator of the nodal signaling cascade, which is essential for the induction of mesoderm during embryonic development.
REFERENCES
1.RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response.Poulsen SL, Hansen RK, Wagner SA, van Cuijk L, van Belle GJ, Streicher W, Wikstrom M, Choudhary C, Houtsmuller AB, Marteijn JA, Bekker-Jensen S, Mailand NJ Cell Biol. 2013 Jun 10;201(6):797-807. doi: 10.1083/jcb.201212075.2.Arkadia, a Novel SUMO-Targeted Ubiquitin Ligase Involved in PML Degradation.Erker Y, Neyret-Kahn H, Seeler JS, Dejean A, Atfi A, Levy LMol Cell Biol. 2013 Jun;33(11):2163-77. doi: 10.1128/MCB.01019-12. Epub 2013 Mar 25.3.RNF111-Dependent Neddylation Activates DNA Damage-Induced Ubiquitination.Ma T, Chen Y, Zhang F, Yang CY, Wang S, Yu XMol Cell. 2013 Mar 7;49(5):897-907. doi: 10.1016/j.molcel.2013.01.006. Epub 2013 Feb 7.4.RB1CC1 positively regulates transforming growth factor-{beta} signaling through the modulation of Arkadia E3 ubiquitin ligase activity.Koinuma D, Shinozaki M, Nagano Y, Ikushima H, Horiguchi K, Goto K, Chano T, Saitoh M, Imamura T, Miyazono K, Miyazawa K.J Biol Chem. 2011 Jul 27. [Epub ahead of print]5.Efficient TGF-beta/SMAD signaling in human melanoma cells associated with high c-SKI/SnoN expression.Javelaud D, van Kempen L, Alexaki VI, Le Scolan E, Luo K, Mauviel A.Mol Cancer. 2011 Jan 6;10(1):2.6.Context-dependent regulation of the expression of c-Ski protein by Arkadia in human cancer cells.Nagano Y, Koinuma D, Miyazawa K, Miyazono K.J Biochem. 2010 Apr;147(4):545-54. Epub 2009 Dec 2.7.Overexpression of snon/skil, amplified at the 3q26.2 locus, in ovarian cancers: a role in ovarian pathogenesis.Nanjundan M, Cheng KW, Zhang F, Lahad J, Kuo WL, Schmandt R, Smith-McCune K, Fishman D, Gray JW, Mills GB.Mol Oncol. 2008 Aug;2(2):164-81. Epub 2008 May 10.8.Arkadia activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.Levy L, Howell M, Das D, Harkin S, Episkopou V, Hill CS.Mol Cell Biol. 2007 Sep;27(17):6068-83. Epub 2007 Jun 25.
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