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>   首页   >   产品   >   一抗   >   其他   >   Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (ATTO 488 Conjugated) Secondary Antibody   

Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (ATTO 488 Conjugated) Secondary Antibody

Rabbit Polyclonal, ATTO 488

     
  • 3 - Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain)  (ATTO 488 Conjugated) Secondary Antibody ASR3259
    ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
  • 1 - Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain)  (ATTO 488 Conjugated) Secondary Antibody ASR3259
    Western Blot of ATTO 488 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: ATTO 488 rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG Subclasses. Other band(s): None.
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Product Information
Description Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody ATTO 488 Conjugated
Host Rabbit
Conjugate ATTO 488
FP Value 3.1 moles ATTO 488 per mole of IgG
Target Species Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (gamma 1, 2a, 2b and 3 chain)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Reconstitution Volume 500 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Application Note The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
Purity Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 488 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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