癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-MOUSE IgG1 (Gamma 1 chain) (ATTO 488 Conjugated) Secondary Antibody
Goat Polyclonal, ATTO 488
- 产品详情
- 实验流程
- 背景知识
| Description | Anti-MOUSE IgG1 (Gamma 1 chain) (GOAT) Antibody ATTO 488 Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins) |
|---|---|
| Host | Goat |
| Conjugate | ATTO 488 |
| FP Value | 3.6 moles ATTO 488 per mole of IgG |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
Application 
| IF, WB |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG1 |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgG1 heavy chain |
| Reconstitution Volume | 500 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Application Note | The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. |
| Purity | Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 0.5% of target signal. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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