Anti-Chicken IgM (mu chain) (Alkaline Phosphatase Conjugated) Secondary Antibody

Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)

Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007) 1:30,000 for 60 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab), 75 kDa for IgM. Chicken F(c) migrates slightly higher.

Product Information
Description	Anti-CHICKEN IgM (mu chain) (GOAT) Antibody Alkaline Phosphatase Conjugated
Host	Goat
Conjugate	Alkaline Phosphatase (Calf Intestine)
Target Species	Chicken
Clonality	Polyclonal
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Physical State	Liquid (sterile filtered)
Host Isotype	IgG
Target Isotype	IgM µ chain
Buffer	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen	Chicken IgM mu heavy chain
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide

Additional Information
Shipping Condition	Wet Ice
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against Chicken IgG.
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.