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Anti-Chicken IgG F(c) (Alkaline Phosphatase Conjugated) Secondary Antibody

Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)

     
  • 1 - Anti-Chicken IgG F(c)  (Alkaline Phosphatase Conjugated) Secondary Antibody ASR2943
    Western Blot of Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and F(ab), 75 kDa for IgM. Chicken F(c) migrates slightly higher.
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Product Information
Description Anti-CHICKEN IgG F(c) (RABBIT) Antibody Alkaline Phosphatase Conjugated
Host Rabbit
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Chicken
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG F(c)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Chicken IgG F(c) fragment
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Application Note This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:9,000 of the reconstitution concentration is suggested for this product.  This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:9,000 of the reconstitution concentration is suggested for this product. 
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum.  No reaction was observed against Chicken IgG F(ab’)2.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Chicken IgG Antibody detects specifically Chicken IgG F(c). Anti-Chicken IgG F(c) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Chicken IgG F(c) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.

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