Anti-Guinea Pig IgG F(c) (Alkaline Phosphatase Conjugated) Secondary Antibody

Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)

Dot Blot of Goat anti-Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Guinea Pig IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.

Product Information
Description	Anti-GUINEA PIG IgG F(c) (GOAT) Antibody Alkaline Phosphatase Conjugated
Host	Goat
Conjugate	Alkaline Phosphatase (Calf Intestine)
Target Species	Guinea Pig
Clonality	Polyclonal
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	DB
Physical State	Liquid (sterile filtered)
Host Isotype	IgG
Target Isotype	IgG F(c)
Buffer	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen	Guinea Pig IgG F(c) fragment
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide

Additional Information
Shipping Condition	Wet Ice
Application Note	This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(ab’)2.
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.