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Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody

Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)

     
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Product Information
Description Anti-CHICKEN IgG (H&L) (RABBIT) Antibody Alkaline Phosphatase Conjugated
Host Rabbit
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Chicken
Clonality Polyclonal
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Chicken IgY / IgG whole molecule
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Application Note Secondary antibodies are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods
Purity Anti-Chicken IgY / IgG Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY / IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgY / IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
Storage Condition Store Secondary Antibody at 4° C before opening. DO NOT FREEZE. This antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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