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F(ab')2 Anti-Mouse IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody
Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)
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| Description | F(ab')2 Anti-MOUSE IgG (H&L) (RABBIT) Antibody Alkaline Phosphatase Conjugated Min X Human Serum Proteins |
|---|---|
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase (Calf Intestine) |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG F(ab')2 |
| Target Isotype | IgG (H&L) |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Immunogen | Anti-Mouse F(ab')2 IgG (H&L) was produced by repeated immunization with Mouse IgG whole molecule in rabbit. |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.1% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Application Note | Antibody Anti-Mouse F(ab')2 IgG (H&L) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins. |
| Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-Mouse F(ab')2 IgG (H&L) antibody generated in rabbit detects specifically mouse F(ab')2 IgG (H&L). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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