Goat Anti-Mouse IgG (H&L)-HRP Polymer

Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) HRP polymer labled

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IC, E
Reactivity	Mouse

Additional Information
Source	Goat
Immunogen	Mouse IgG
Purification	Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse, rabbit and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Target/Specificity	By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG. No antibody was detected against non immunoglobulin serum proteins.
Clonality	Polyclonal
Format	0.5 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.05% Proclin
Dilution	E (1/5000 - 1/20000), WB (1/5000 - 1/20000), IH (1/100 - 1/500), IC (1/100 - 1/500)
Storage	Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.