Anti-GCGR Reference Antibody (crotedumab)

Recombinant Antibody

Anti-GCGR Reference Antibody (crotedumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-GCGR Reference Antibody (crotedumab)is more than 96.61% ,determined by SEC-HPLC.
Human GCCR His EGFP HEK293 cells were stained with Anti-GCGR Reference Antibody (crotedumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC668=0.6028 µg/mL

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P47871
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Isotype	IgG4SP
Calculated MW	54009 Da

Additional Information
Target/Specificity	GCGR
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	GCGR
Function	G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
Cellular Location	Cell membrane; Multi-pass membrane protein. Note=Is rapidly internalized after ligand-binding

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.